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A) Overview of <t>CRISPR</t> screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 <t>CRISPRa</t> screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.
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A) Overview of <t>CRISPR</t> screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 <t>CRISPRa</t> screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.
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A) Overview of <t>CRISPR</t> screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 <t>CRISPRa</t> screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.
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A) Overview of <t>CRISPR</t> screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 <t>CRISPRa</t> screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.
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A) Overview of CRISPR screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 CRISPRa screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.

Journal: bioRxiv

Article Title: A whole genomic CRISPR-Cas9 screen identifies the amino acid transporter SLC43A1 (LAT3) as a major determinant of oxaliplatin sensitivity in colorectal cancer cells

doi: 10.1101/2025.04.21.649594

Figure Lengend Snippet: A) Overview of CRISPR screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 CRISPRa screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.

Article Snippet: The Human Calabrese CRISPR Activation Pooled Library Set A was a gift from David Root and John Doench (Addgene viral prep #92379-LVC) ( ).

Techniques: CRISPR, Control, Labeling